DNA-based detection of Leishmania and Crithidia species isolated from humans in cutaneous and post-kala-azar dermal leishmaniasis from Shiraz and Kharameh, southern Iran
نویسندگان
چکیده
منابع مشابه
Kala-azar and Post–Kala-azar Dermal Leishmaniasis, Assam, India
remain unknown. S. delphini has rarely been identified in horses, but, as we observed, it may be misidentified by conventional methods. Although colonization or contamination appeared most likely in the instances we describe, these findings suggest that this opportunistic pathogen can be found in horses and might be pathogenic in certain situations. Our findings highlight the importance of usin...
متن کاملDevelopment of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis.
We have developed a PCR assay that is capable of amplifying kinetoplast DNA (kDNA) of Leishmania donovani in a species-specific manner among Old World leishmanias. With Indian strains and isolates of L. donovani the assay was sensitive enough to detect kDNA in an amount equivalent to a single parasite or less. The extreme sensitivity of the assay was reflected in its ability to detect parasite ...
متن کاملDermoscopy of Post Kala-Azar Dermal Leishmaniasis
© 2018 Indian Dermatology Online Journal | Published by Wolters Kluwer Medknow Post Kala‐azar dermal leishmaniasis (PKDL) is a late cutaneous manifestation of untreated or partially treated visceral leishmaniasis (VL) and is caused by the same organism, i.e., Leishmania donovani. It characteristically manifests as macules, nodules, plaques, and facial erythema. A 19‐year‐old male presented with...
متن کاملDNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans.
Leishmania donovani in India causes visceral infection (kala-azar) and dermal infection (post-kala-azar dermal leishmaniasis). We report here the identification of polymorphism in a well-defined genetic locus among the Leishmania parasites causing the visceral and dermal manifestations, in a comparison of 15 post-kala-azar dermal leishmaniasis and 12 kala-azar patient isolates.
متن کاملExtensive Post-Kala-Azar Dermal Leishmaniasis
© 2018 Indian Dermatology Online Journal | Published by Wolters Kluwer Medknow A 16‐year‐old male from Bihar had confluent hypopigmented plaques along with erythematous succulent papules on the chin, trunk, and shoulders for the past 8 years. There was relative sparing of axillae, suprapubic, and inguinal regions [Figures 1a‐c]. He had a history of kala‐azar. Skin biopsy and smear showed numero...
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ژورنال
عنوان ژورنال: Journal of Vector Borne Diseases
سال: 2020
ISSN: 0972-9062
DOI: 10.4103/0972-9062.309518